
Laser-activated perfluorocarbon nanodroplets (PFCnDs) are emerging phase-change contrast agents that showed promising potential in ultrasound and photoacoustic (US/PA) imaging.
Novel in their simplicity, these methods may promote the use of PFCnDs among a broader user base to study a variety of extravascular phenomena. These data indicate that, through a facile synthesis process, it is possible to produce monodisperse, small-sized PFCnDs. Finally, we highlight the ability of this approach to facilitate US/PA imaging in a murine model of breast cancer. Furthermore, our imaging studies revealed that nanodroplets with more PEGylated lipids produce increased US/PA signal compared with those with the standard formulation.
Our results suggest that increasing the molar percentage of PEGylated lipid reduces the size and size variance of PFCnDs. We investigated the impact of variable shell composition on PFCnD size and US/PA image properties. Producing consistently small, monodisperse PFCnDs remains a challenge without resorting to technically challenging methods.
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